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2×热启动PCR预混液

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MonAmp™ 2× HS Taq Mix的浓度为2×,使用方便快捷,能减少PCR操作过程中的污染,使用时只需取适量MonAmp™ 2× HS Taq Mix,加入模板和引物,并加入ddH2O补足体积,使反应体系浓度为1×即可进行PCR反应。 Mix中包含热启动MonTaq-HS DNA Polymerase,在PCR反应开始前,该酶的聚合酶活性受到抑制,防止低温条件下非特异性退火或引物二聚体导致的非特异性扩增。该Mix具有良好的扩增特异性和模板兼容性,最长可扩增5 kb DNA片段,PCR产物3'端带A碱基,纯化后可直接用于T/A克隆。

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