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快速内切酶AscI

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5'... G G/C G C G C C ...3' 3'... C C G C G C/G G ...5' 快速内切酶,可在 5~15 min 内完成反应 反应温度为37℃ 受CpG甲基化影响,序列完全重叠,剪切阻断 失活条件为80℃ 温育20 min 3 h 温育未表现星号活性,延时酶切可能出现星号活性 同裂酶:PalAI,SgsI

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