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快速内切酶StuI

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5'... A G G/C C T ...3' 3'... T C C/G G A ...5' 快速内切酶,可在5~15 min内完成反应 反应温度为37℃ 受Dcm甲基化影响,序列可能重叠,剪切阻断 受EcoBI甲基化影响, 序列可能重叠,剪切可能受影响 失活条件为80℃温育20 min 3 h温育未表现星号活性,延时酶切可能出现星号活性 同裂酶:AatI,Eco147I,PceI,SseBI

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