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快速内切酶SmaI

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5'... C C C/G G G ...3' 3'... G G G/C C C ...5' 快速内切酶,可在5~15 min内完成反应 反应温度为25℃ 受CpG甲基化影响,序列完全重叠,剪切阻断 失活条件为80℃温育20 min 3 h温育未表现星号活性,延时酶切可能出现星号活性 * 37℃反应时酶活性会略降低,需适当延长温育时间

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