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快速内切酶SbfI

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5'... C C T G C A/G G ...3' 3'... G G/A C G T C C ...5' 快速内切酶,可在5~15 min内完成反应 反应温度为37℃ 失活条件为80℃温育20 min 3 h温育未表现星号活性,延时酶切可能出现星号活性 同裂酶:SdaI, Sse8387I

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