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快速内切酶BglII

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5'... A/G A T C T ...3' 3'... T C T A G/A ...5' 快速内切酶,可在5~15 min内完成反应 反应温度为37℃ 受EcoBI甲基化影响,序列可能重叠,剪切受阻 失活条件为 80℃温育20 min 3 h温育未表现星号活性,延时酶切可能出现星号活性

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